Triacontanol - As a Growth Stimulant.

Looking for something to read along with me? Check out this older report on triacontanol. You can play with this stuff naturally by using our alfalfa sprouting seeds and use of SST methods.

By D.L Hinerman, M.D. and S.L. Kunkel, Ph.D., Ann Arbor, Michigan

For many years beneficial effects of alfalfa on plants, animals and humans have been suspected. In the mid-seventies intense interest was generated by Stanley K. Ries et. al. when they observed significant increases in growth and productivity of plants which were in close proximity to alfalfa hay (Medicago sativa L). Further investigation led to the isolation of the active principle in alfalfa, 1-triacontanol. Alfalfa and triacontanol in small quantities not only stimulated growth and productivity of plants, but did so in the dark independently of photosynthesis.1,3,4,5,6,7,8
       1-Triacontanol (CH3CH28CH2OH) is a ubiquitous long chain alcohol found incorporated in the surface of many plants and is a major component of beeswax.

See Experiment #1 on Rats here --> Full Article Link

Experiment #2 — Growth of Plants

       Although some scientists and plant growers have seen remarkable increases in growth and production of plants from alfalfa and triacontanol, subsequent field tests by Ries and by other scientists from the U.S.D.A., universities and chemical companies have proved inconsistent and disappointing.5 Ries attributes most of the problems to the methods of formulation of triacontanol in that the long chain alcohol is very insoluble in water and in most other solvents. Ries and others have used combinations of solvents, detergents and surfactants in efforts to keep triacontanol in solution. The authors had a batch of triacontanol that had been opened for several months and this sample defied all attempts to get it in solution. A.J. Welebir is reported to have found additional requirements such as a solution at a pH of 8 or higher and the presence of metal ions such as calcium in the triacontanol solution. Using a solution of triacontanol with the above additions, Welebir has produced even more amazing growth and production than others have achieved and has done it consistently.5
       Materials for experiment #2 consisted of eight first-year rooted cuttings (clones) of genetically identical plants of W. Delp's hybrid rhododendron (R. pink carolinianum x R. scintillans) #1. The cuttings were about the same size, consisting of one or two stems, small leaves and fairly uniform small root systems. They were placed in a rooting bed in a cold greenhouse (minimum temperature was 40°F.) The soil mixture was equal parts of peat and perlite. Bottom heat of 80°F was present. One mg of 1-triacontanol was dissolved in one ml of chloroform, carefully diluted with de-ionized water to make a 0.1 mg/liter solution. (Ries had found that aqueous solutions containing small amounts of chloroform had no effect on plants.) The pH of the solution was 5.3. The pH of the rooting mixture was 5.5. No calcium ions were present except in buffers found in the well-water (pH 7) with which the plants were sprayed at frequent intervals.
       The trial began in mid-January when plants were dormant. Six of the eight plants were painted thoroughly with the triacontanol solution and this was repeated in one week and again in mid-April. In May the rooted cuttings were transplanted to individual pots. In June only four of the 8 plants were sprayed and in August only 2 of the 8 plants were sprayed making 5 the maximum number of doses for two of the plants.
       Figure II illustrates the difference in growth between the total controls and the plants receiving 5 treatments. Five different measurements of growth were taken in early October.

Figure II Measurements of five different aspects of growth of rooted cuttings of a rhododendron hybrid, both untreated and treated with 0.1 mg triacontanol/liter.
Total Weight (minus soil) 30.2 g 83.1 g 275%
Diameter of trunk at base 4.0 mm 8.0mm 100%
Number of branches 4 10 150%
Number of leaves 62 167 269%
Number of flower buds 4 8 100%

       The first results of the experiment was an obvious premature break in dormancy within the first two weeks in the triacontanol treated plants (end of January), whereas dormancy did not end until early May in the control plants. After 2 months the growth in treated plants was double that of the untreated plants. At the end of the growing season the amount of growth roughly paralleled the number and duration of treatments. A fantastic amount of growth had occurred especially in those plants which had 4 and 5 treatments over most of the growing season. Those receiving 5 treatments had tertiary branching as compared to secondary branching in those with 3 and 4 treatments and primary branching in the remainder. The root system after 5 treatments appeared proportionately larger than other aspects of the plant. One of the most profound differences was that of a total weight of 2¾ times greater in maximally treated plants as compared to untreated plants. Of great interest also was the double number of blossom buds in maximally treated plants and the diameter of those buds was twice that of the untreated plants.

Comparison of treated and untreated plants.
   Figure III  Photograph illustrating a control or untreated 
plant on the left side compared with two triacontanol treated
plants (center and right side) of identical rooted cuttings
of (R. pink carolinianum x R. scintillans) #1. The plant in
the center was treated five times through August; the
plant on the right was treated four times through June.

Results of these two studies indicated that 1 -triacontanol can act as a powerful plant growth stimulant; yet this long chain lipid alcohol appears to have no stimulatory effect on mammalian DNA increase, since there was no increase in the uptake of 3H-thymidine by mouse spleen cells. Of course the Environmental Protection Agency must approve triacontanol before it can be used commercially. At least we can use it in trials with the knowledge that it probably will not influence the growth of cells in man and animals.
       Scientists have been mystified by the mechanism of action of triacontanol on plants. We have nothing to add to solve the mystery of why the addition of trace amounts of triacontanol to a plant which already had much greater amounts of this chemical will produce doubling or tripling of growth in some instances. Why or how does it increase flowering and productivity? How does triacontanol replace light, produce growth in the dark and break dormancy in plants? All of these are highly desirable qualities.
       Crop production, like blossom and seed production, is dramatically increased and at trifling cost. Rooting of cuttings and seedling production is enhanced.
       It is obvious that the proper use of 1-triacontanol and other newly discovered natural substances will revolutionize the plant and farming industries. The world-wide mass starvation of people, which has been predicted because of our destruction of farmland, may be delayed.
       Nurserymen can produce larger, healthier and more mature plants in much less time and less expense than at present, also they will be able to force a greater variety of plants with more numerous and larger flowers than formerly possible. These changes do not appear to reduce hardiness in the plants. These are but a few examples of changes which will guarantee a revolution in the plant industry with the use of these new plant growth regulators.

1. Bittender, H.C.; Dilley, D.A.; Wert, V. and Ries, S.K.: Environmental Parameters Affecting Dark Response of Rice Seedlings (oryza sativa L) to Triacontanol. Plant Physiol. 61:851-854, 1978
2. Cave, M.D.: Incorporation of Tritium-labeled Thymidine and Lysine into Chromosomes of Cultured Human Leukocytes. J. Cell Biol. 29:209-220, 1966
3. Hangarter, R.; Ries, S.K. and Carlson, P.: Effect of Triacontanol on Plant Cell Cultures In Vitro. Plant Physiol. 61:855-857, 1978
4. Hinerman, D.L.: Stimulation of Growth by Alfalfa. Quarterly Bulletin. American Rhododendron Society 33:70-73, 1979
5. Maugh, T.H., II. New Chemicals Promise Larger Crops. Science 212:33-34, 3 April, 1981
6. Jones, J.; Wert, V. and Ries, S.K.: Specificity of 1-Triacontanol as a Plant Growth Stimulator and Inhibition of its Effects by other Long Chain Compounds. Planta 144:277-282, 1979
7. Ries, S.K.; Wert, V.F.; Sweeley, C.C. and Leavitt, R.A.: Triacontanol: A New Naturally Occurring Plant Growth Regulator. Science 195:1339-1341, 25 March, 1977
8. Ries, S.K.; Richman, T.L.; Wert, V.F.: Growth and Yield of Crops Treated with Triacontanol. J. Amer. Soc. Hort. Sci. 103(3):361-364, May 1978

We express thanks to Mrs. Robin Kunkel for preparing the chart, graph and photograph in a pleasing manner, Mrs. Marjorie Owens for secretarial assistance and Mr. Craig Biddie and Mr. Eddie Burks for the photographs.



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